<i>sip1-62</i> mutant cells exhibit immunosuppressant-, micafungin-, and temperature-sensitive phenotypes similar to those of <i>sip1-i4</i> mutant cells.

<p>(A) Immunosuppressant, micafungin, valproic acid and temperature sensitivities of <i>sip1-62</i> mutant cells. Wild-type cell (wt), <i>sip1-i4</i> mutant cells (<i>sip1-i4</i>), and <i>sip1-62</i> mutant cells (<i>sip1-62</i>) were streaked onto plates containing YES or YES plus 0.5 µg/mL FK506, 0.5 µg/mL micafungin, or 6 mM valproic acid, followed by incubated at 27°C for 4 d or at 36°C for 3 d. (B) The <i>sip1-i4</i> mutant cells and <i>sip1-62</i> mutant cells are defective in cytokinesis. Wild-type cell (wt), <i>sip1-i4</i> mutant cells (<i>sip1-i4</i>), and <i>sip1-62</i> mutant cells (<i>sip1-62</i>) were incubated at 25°C for 1 h, at restrictive temperature (36°C) for 1 h, or FK506 was added for 1 h after the cells were incubated at 25°C for 5 h, and then stained with calcofluor to visualize cell wall and septum. Arrowheads indicated the multi-septated cells. Bar, 10 µm. (C) Septation index and percentage of multi-septated cells in wild-type cell (wt), <i>sip1-i4</i> mutant cells (<i>sip1-i4</i>), and <i>sip1-62</i> mutant cells (<i>sip1-62</i>). Cells in C were incubated as in B. The data represent means ± standard deviations of three independent experiments. At least 50 cells were observed at one time in each experiment.