Colocalization of Bgs1 with FM4-64 in wild-type, <i>sip1-i4</i> mutant, and Δ<i>apm1</i> cells.

<p>(A) Bgs1 did not localize at cell ends, although it partially accumulated in the Golgi/endosomes in <i>sip1-i4</i> mutant and Δ<i>apm1</i> cells. Wild-type (wt), <i>sip1-i4</i> mutant (<i>sip1-i4</i>), and Apm1-deletion cells (Δ<i>apm1</i>), which expressed chromosome-borne GFP-Bgs1, were examined by fluorescence microscopy under the repressed conditions. Cells were cultured in EMM at 27°C for 6 h. GFP-Bgs1 localization and FM4-64 fluorescence were examined under a fluorescence microscope. Arrowheads indicate the dot-like structures of GFP-Bgs1 and Golgi/endosomes stained with FM4-64; double arrowheads indicated cytoplasmic accumulation, and arrows highlight the concentrated fluorescence at the medial region and cell ends. Bar, 10 µm. (B) Percentage of cells in which Bgs1 was localized at cell ends in wild-type (wt), <i>sip1-i4</i> mutant (<i>sip1-i4</i>), and Apm1-deletion cells (Δ<i>apm1</i>). (C) Quantitative analysis of the number of Bgs1 dots co-localizing with FM4-64/cell in wild-type (wt), <i>sip1-i4</i> mutant (<i>sip1-i4</i>), and Apm1-deletion cells (Δ<i>apm1</i>). Cells in B and C were incubated as in A. The data represent means ± standard deviations as the average of three independent experiments with 150 cells in B and C.