Persistence of epidemic strain of <i>V. cholerae</i> N16961 in filter sterilized lake water (FSLW) microcosms stored statically at room temperature.

<p>(A) persistence of two microcosms (M4 and M12) for >700 days. (B) persistence of <i>V. cholerae</i> in transfer (daughter) microcosms. Transfer microcosm (TM) was prepared by transferring one ml culture from original microcosm (M4, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045187#pone-0045187-g001" target="_blank">Figure 1A</a>) to fresh FSLW (49-ml) at day 96. The microcosms were stored statically at room temperature. Data represent the persistence of three microcosms in excess of 600 days.