The regulation of T cell surface activation markers is unaffected in <i>dlg1</i> knockout mice.

<p>(A) Splenocytes isolated from <i>dlg1^wt;GT</i> (black) or <i>dlg1^ko;GT</i> (red) mice were activated with indicated concentrations of plate bound anti-CD3 in the presence of anti-CD28 for 24 hours and assessed for the expression of activation markers. Histograms were gated on viable donor derived (CD45.2⁺) CD4⁺ or CD8⁺ T cells and show one representative mouse per genotype (n≥3). (B) Flow cytometry profiles of selected surface markers on purified CD4⁺ T cells from <i>dlg1^flox/flox</i> (top <i>panel</i>) or <i>dlg1^flox/flox:CD4^cre</i> (<i>bottom panel</i>) mice stimulated with T-depleted splenocytes with (unshaded) or without (shaded) anti-CD3 antibody for 24 hrs (n≥3).