GSEA analysis of microarray data showing enrichment of the JAK-STAT and MAP Kinase pathways and upregulation of ECM structural component genes along with qRT-PCR validation of the down-regulation of topoisomerase 2 gene expression in MCF7VP cells.

<p>Fig. 5A. Gene set enrichment analysis of microrray data depicting the enrichment of genes in the JAK-STAT signaling pathway. The GSEA software was used to calculate the enrichment levels. Fig. 5B. Gene set enrichment analysis of microrray data depicting the enrichment of genes in the MAP kinase signaling pathway. The GSEA software was used to calculate the enrichment levels. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045268#pone-0045268-g005" target="_blank">Figure 5C</a>. Gene set enrichment analysis depicting enrichment of ECM (extra-cellular matrix) genes in MCF7VP cells. The GSEA algorithm was used to calculate the enrichment levels. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045268#pone-0045268-g005" target="_blank">Figure 5D</a>. Down-regulation of TOPO2A (the drug target of etoposide) in MCF7VP cells. The microarray data was validated by qRTPCR as depicted in the bar chart which shows differences in fold change.