Immunohistochemistry of the ciliary body.

<p>Immunohistochemistry of eight proteins involved in the four major functionalities of the ciliary body. For the progenitor cell properties, we stained FGFR3 and NOTCH1. For the neural background, we stained ROBO1 and GRIN2C. We chose EDNRB and MNAR for the endocrine signaling function, and SERPING1 and TLR3 as examples for the immunological functions. Red staining is the secondary antibody with Cy3 that binds to the specific primary antibody, indicated in the figures. Blue staining is Dapi which stains the cell nucleus. Green staining is FITC channel to look for autofluorescence. We found minimal autofluorescence. One negative control (blanco) for ROBO1 is shown. For the other antibodies, the negative controls were also negative (not shown). We found staining for all proteins in both the non-pigmented (NPE) and pigmented epithelium (PE). The white scale bare indicates 20 µm. The symbol * indicates NPE and x indicates PE.