Shear stress adaptation and actin reorganization.

<p><b>a:</b> Time dependent change of average FRET ratio in the upper cell in (<b>d</b> and <b>e</b>), showing that changes in actinin stress occur in three distinct phases under shear stress of 0.74 dyn/cm². <b>b:</b> Typical variation of average FRET ratio for cell under static condition in the chamber. <b>c:</b> Statistics of FRET peak changes for three phases (n = 7) and variation under static conditions (n = 8) (red, left axis); and statistics of time period for three phases and to saturation (n = 7) (gray, right axis). (*) indicates peaks observed under flow. <b>d,e:</b> Respective FRET ratio and CFP images of two cells subjected to shear stress at times indicated in (a). The FRET ratio is shown in 16 color map, where blue indicates a low FRET ratio and higher tension; red indicates a higher FRET ratio and lower tension. The numbers in brackets indicate the duration under flow. The arrow on the left of (d) indicates flow direction. The scale bar represents 10 µm. <b>f, g:</b> Time dependent FRET distribution along the window in (d), and time dependent actin intensity along the window in (e). These distributions are correlated with various phases in (a), which is also replotted for ease of discussion.