Effect of mechanosensitive channel inhibitors on flow-induced cytoskeleton reorganization.

<p>Immunostained F-actin in MDCK cells under <b>a:</b> static condition and <b>b:</b> shear stress of 0.74 dyn/cm² for 3 hrs. <b>c:</b> Statistics of actin arrangements under static (red) and shear stress (green). The percentage was estimated for each experiment, and the error bars show standard errors of “n” experiments (static, n = 7; shear flow, n = 10). <b>d:</b> F-actin staining in the presence of 100 µM Gd³⁺ in cells in static condition and <b>e:</b> under shear flow of 0.74 dyn/cm² for 3 hrs, showing Gd³⁺ inhibits flow induced cytoskeleton reorganization. <b>f:</b> Statistics of actin arrangements of (d) vs (e) (static+Gd³⁺: n = 4; flow+Gd³⁺: n = 8). The scale bar is 20 µm.