Effect of shear stress on cell motility.

<p><b>a–c:</b> Trace of single cell movement tracked by centroid of the nucleus for 2.5 hrs under <b>a:</b> static condition; <b>b:</b> shear stress of 0.74 dyn/cm²; and <b>c:</b> same flow condition with addition of 100 µM Gd³⁺. The cell's original location (defined by the center of nucleus) is at (0,0) and the motion is tracked along the x and y axis every 16 min for a total ∼160 min. The arrows in <b>b</b> and <b>c</b> indicate flow directions. <b>d:</b> CFP images showing the typical movement of a cell under static condition. The yellow stars in (d) indicate the center position of nucleus that was traced and plotted in (a). The scale bar represents 10 µm.