Mouse PLT depletion abolished hPLT sequestration in the lungs of LPS primed SCID mice.

<p><b>A</b>: Confocal images of mouse lung or liver sections. GPIb antibody treatment abolished hPLT sequestration in the lungs of septic SCID mice (A-c,d vs. a, b), visualized by anti-hCD41 antibody staining. Yet GPIb antibody treatment did not abolish platelet accumulation in the liver (A-g, h vs e, f). Neutrophil count, visualized by endogenous LYS-eGFP fluorescence, in the lung was similar before and after PLT depletion (A-k vs i, l vs j). The composite images shown were representative of 5 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. <b>B</b>: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse lung sections shown in A-a to d. <b>C</b>: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse liver sections shown in A-e to h. Data are expressed as means ± SD; n = 5. *p<0.05;**p<0.01.