The <i>miR-106b-93-25</i> cluster promoted EMC cell growth.

<p>(<b>a</b>)At indicated times post-transfection, MTT assays were used to compare the effects of <i>miR-106b</i>, <i>miR-93</i>, <i>miR-25</i> and their inhibitors. <i>miR-106b</i>, <i>miR-93</i>, <i>miR-25</i> promoted the growth of ECC-1 cells, while their inhibitors significantly decreased proliferation in ECC-1 cells. Values represent the means ± SD from triplicate determinations. (<b>b</b>) Effects of <i>miR-106b</i>, <i>miR-93</i> or <i>miR-25</i> on the cell cycle. FACS analysis was performed on cells transfected with either miRNA or miRNA inhibitors. <i>miR-106b</i> and <i>miR-93</i> gain of function led to an increase in cells in S-phase, while the G1-phase population was increased in the cells transfected with their inhibitors. miR-25 gain of function had no effects on the cell cycle. (<b>c</b>) Effects of <i>miR-106b</i> or <i>miR-93</i> or <i>miR-25</i> on apoptosis. FACS analysis was performed on cells transfected with either miRNA or miRNA inhibitors. miR-25 gain of function decreased apoptosis of cells. Apoptosis increased significantly in cells transfected with the <i>miR-25</i> inhibitor. <i>miR-106b</i> and <i>miR-93</i> had no effect on the apoptosis levels of the cells.