Adipogenic potency of SVSC and IVSC.

<p>The overall experimental scheme is illustrated in A. SVSC and IVSC differentiation was induced at d0, the cells (B-E, J represent SVSC; F-I, K represent IVSC; B-D and F-H, 100×; E and I 400×) were fixed and stained with oil red O at d3 (B; F,), d6 (C; G,) and d9 (D, E, J; H, I, K). Triglycerides contents in SVSC and IVSC in growth medium (GM) and differentiation medium (MDI) at d10 were also determined. Replicates were the same as L (L). Adipogenic-related mRNAs and miRNAs were quantified by qPCR at different times after MDI stimulation (M and N, respectively). Date were first normalized to <i>GAPDH</i> mRNA for each mRNA and miRNA, then fold changes relative to their respective levels in IVSC preadipocyte (day -2) were calculated, replicates were the same as L. C/EBPα protein levels during SVSC and IVSC differentiation were determined by western blotting, GAPDH protein was used as endogenous control (O).