Molecular characterization of papillomas from WT, stathmin heterozygous and KO mice.

<p>(A) DNA sequence analysis, using the Sanger method, of H-Ras in tumors from WT, stathmin heterozygous and KO mice. The T-A substitution in heterozygosis is framed in black. A typical sequence is reported. (B) qRT-PCR analyses of K-Ras4a, K-Ras4b, c-Fos, Egr-1, Jun-B, Cyclin D1 and c-Myc mRNA expression in tumors from mice of the indicated genotype. The horizontal bar within the box indicates the median expression of each gene. (C) Western blot analyses of AKT and MAPK activation in papillomas derived from mice of the indicated genotype. On the right, the box-plot show the ratio between phosphorylated and total ERK (upper panel) and phosphorylated and total AKT (lower panel), in 6 tumors/genotype. <i>p = n.s.,</i> using the Mann Whitney test.