<i>Sb</i> secretes several molecules that differentially regulate both cell adhesion and migration.

<p>(A) Isolated HCT-8/E11 cells were treated with or without <i>Sb</i>S (<i>Sb</i>S), boiled <i>Sb</i>S (<i>Sb</i>S boiled) and plated on 3.15 µg/ml vitronectin. Cell-vitronectin adhesion was evaluated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s2" target="_blank">Methods</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s3" target="_blank">Results</a> are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. (B) Isolated HCT-8/E11 cells were treated with or without <i>Sb</i>S (<i>Sb</i>S), or fractionated <i>Sb</i>S (>9 kDa or <9 kDa) and plated on 3.15 µg/ml vitronectin. Cell-vitronectin adhesion was evaluated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s2" target="_blank">Methods</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s3" target="_blank">Results</a> are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. (C) HCT-8/E11 cell monolayers were wounded and incubated without (Ctrl) or with <i>Sb</i>S (<i>Sb</i>S), boiled <i>Sb</i>S (<i>Sb</i>S <i>boiled</i>) or fractionated <i>Sb</i> supernatant (>9 kDa or <9 kDa) for 5 h. Wound closure was determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s2" target="_blank">Methods</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s3" target="_blank">Results</a> are expressed as the percentage of cell migration compared to control. Data represent the mean+SD of 2 separate experiments. *** P<0.001.