Blood perfusion imaging and function assessment of ischemic hindlimb.

<p>Laser Doppler perfusion imaging (LDPI) visualized dynamic changes in hindlimb perfusion (<b>a</b>). Colored scale bar represents blood flow velocity in LDPI index. Blood perfusion was quantified using perfusion ratio (PR), i.e., the ratio of average LDPI index of ischemic (red arrows) to nonischemic hindlimb (<b>b</b>). Representative photos show hindlimb autoamputation or salvage (yellow arrows) among groups on day 21 (<b>c</b>). Significantly lower autoamputation percentage (<b>d</b>), scores of ischemic damage (<b>e</b>) and ambulatory impairment (<b>f</b>) were observed in the ADSC group compared to the control group. n = 20 for each. Error bars: mean±SD. *<i>P</i><0.001 <i>vs.</i> Control, #<i>P</i><0.05 <i>vs.</i> Control, †<i>P</i><0.001 <i>vs.</i> Control.