Plasmin activity in the conditioned media.

<p>(A) Western blot analysis for detection of plasmin(ogen) with a monoclonal anti-human plasminogen antibody, in conditioned medium obtained from the VG and N samples of 2 representative patients (Pn and Pg: purified human plasmin, 0.25 µg <i>per</i> well, and purified human plasminogen 0.2 µg <i>per</i> well, respectively, used as reference). Positions in the gel of molecular mass standard proteins used for calibration are indicated on the left. (B) Plasmin activity quantified in the conditioned media of N and VG, using a selective chromogenic substrate (<i>P</i> = 0.8).