Estimated concentration of GAGE proteins in melanoma cells.
- Morten F. Gjerstorff (133618)
- Heike I. Rösner (133621)
- Christina B. Pedersen (133623)
- Katrine B. V. Greve (133624)
- Steffen Schmidt (133628)
- Katherine L. Wilson (133633)
- Jan Mollenhauer (133637)
- Hüseyin Besir (41778)
- Flemming M. Poulsen (133642)
- Niels Erik Møllegaard (8834)
- Henrik J. Ditzel (102536)
Publication date
September 2012
DOIAbstract
<p>A. Dot blots to estimate GAGE protein content in MZ2-MEL and SK-MEL-31 whole cell lysates by comparison to recombinant GAGE12I. Blots were probed with antibodies expected to recognize all GAGE family members; the number below each dot indicates the relative signal intensity. B. Relative quantification of GAGE proteins in nuclei and cytoplasm of MZ2-MEL and SK-MEL-31 cells based on immunostaining intensities of confocal images measured using ImageJ64 software.</p
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