FRET efficiencies of native and unfolded doubly dye-labeled apoflavodoxins.

<p>Fluorescence emission intensity is of donor (A488) at 515 nm with excitation at 450 nm. Fluorescence of native protein is determined at 0.1 M GuHCl (i.e., at the lowest concentration denaturant used), whereas fluorescence of unfolded protein is determined at 6.9 M GuHCl.