Vaccination-challenge analysis of the immunogenic potential of Z234.

<p>C57BL/6 mice were vaccinated with PBS (n = 4; open symbols), <i>ssaV</i> (5×10⁷ cfu; n = 9; grey) or Z234 (5×10⁷ cfu; n = 13; black). 40 days post immunization (PBS (n = 4); M2735 (n = 5); Z234 (n = 7)), mice were ampicillin-treated (20 mg by gavage) and challenged with wt SB300 (amp^r, sm^r) for two days.. Colonization of cecum (A), MLN (C), Spleen (D) and cecal pathology (B) after wt <i>S</i>. Typhimurium, day 2 post challenge was shown accordingly. Disease parameters (vaccination control data) were determined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045433#pone.0045433.s003" target="_blank">Figure S3B</a>. Striped line: detection limit.