Thermodynamic characterization of monomeric and dimeric MpNep2 by chemical and physical agents.

<p><b>A</b>. Urea denaturation plots obtained by monitoring Trp fluorescence emission and calculating as degree of denaturation (<i>α</i>) from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045620#pone.0045620.e001" target="_blank">equations 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045620#pone.0045620.e002" target="_blank">2</a> (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045620#s2" target="_blank">Material and methods</a>). Inset shows linear regression of versus urea. <b>B</b>. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer that do not contain urea. <b>C</b>. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer containing the corresponding urea concentration depicted on legend. <b>D</b>. Thermal denaturation plots obtained by monitoring Trp fluorescence emission and calculating degree of denaturation. A and D – Experiments are shown as mean (n = 3) with independent protein preparations. B and C – UFLC was performed on Superdex 75 and Superdex 200, respectively at 0.7 mL.min⁻¹.