Age-dependent accumulation of tau protein in soluble and insoluble protein fractions in 3xTg-AD mice.

<p>Western immunoblots showing tau accumulation with age as quantified in the TBS-soluble (soluble proteins) and the formic acid-soluble (insoluble proteins) fractions from the frontal cortex of 3xTg-AD mice. Consistent trends toward increases were seen in female 3xTg-AD mice. (<b>E–H</b>) To evaluate the relation between tau load and social behaviour, the number of social events displayed by 3xTg-AD mice (relative to its NonTg partner) was plotted against soluble tau concentrations in the frontal cortex. A significant correlation could only be established between insoluble tau concentrations and social events in female across the 2 groups of age (dotted lines).