Effect of 11,12-EET on the mitochondrial membrane potential.

<p>(A) Representative tracing of the 11,12-EET (3 µmol/L)-induced changes in JC-1 fluorescence in pulmonary artery endothelial cells from wild-type (WT) cells in the absence or presence of iberiotoxin (IbTx, 300 nmol/L) and from BKβ₁^−/− cells. (B) Mitochondrial membrane depolarization by 11,12-EET in WT and BKβ₁^−/− pulmonary artery smooth muscle cells in the presence of solvent (Sol) or IbTx. (C) Mitochondrial membrane depolarization by 11,12-EET in WT cells in the presence of Sol, 14,15-EEZE (10 µmol/L), or Rp-cAMPS (10 µmol/L). All experiments were performed in the presence of diclofenac (10 µmol/L) and L-NA (300 µmol/L). The bar graphs summarize data obtained in 4–10 independent experiments; *P<0.05, ***P<0.001 versus WT+EET.