Lack of p50 increases DC survival.

<p>(A) Cytofluorimetric analysis of apoptotic BM-DC upon LPS stimulation. Data represent mean ± SEM (N = 7) (B) Cytofluorimetric analysis of splenic DC survival 24 h and 48 h after LPS injection in mice. Data represent mean ± SEM of 3 independent experiments (each dot corresponds to 1 mouse). (C) Analysis of MHC II⁺ splenic DC survival 24 h after the injection of 100 ng LPS/g (animal weight). <i>In situ</i> apoptosis of splenic DC was determined by TUNEL staining. Results are representative of 3 independent experiments (4 mice/group). (D) Bax protein levels in wt and p50^−/− BM-DC stimulated with LPS for the indicated time. Left panel, 1 of 2 independent experiments is shown. Right panel, mean ± SEM (N = 2). (E) <i>In vitro</i> BM-DC migration of wt and p50^−/− BM-DC. 24 h LPS stimulated BM-DC were seeded in a boyden chamber and migration towards CCL-19 was evaluated after 90′ incubation. (F) <i>In vivo</i> migration of wt and p50^−/− BM-DC to draining lymph nodes. BM-DC unstimulated or stimulated with LPS for 24 h were labeled with a fluorescent dye and injected into the footpads of wt mice. Popliteal draining lymph nodes were recovered 24 h and 48 h later and percentage of migrated BM-DC was evaluated by flow cytometry (N = 4−15). *<i>P</i><0.05 <b>**</b><i>P</i><0.01, <i>t</i> test.