Characterization of the CD44^low, CD44^high/EGFR^high and CD44^high/EGFR^low subpopulations within the LK0923 cell line.

<p><b>A:</b> Expression of CD44 and epidermal growth factor receptor (EGFR) in sorted subpopulations (β-actin was used as a loading control). <b>B:</b> Proliferation rate of subpopulations. Data represents mean ± SD from three experiments in triplicate, *p≤0.05. <b>C:</b> Cell cycle distribution comparison between CD44^low and CD44^high/EGFR^low cells. Data from one representative experiment out of two are shown. <b>D:</b> Plating efficiency, as assessed by colony formation in sorted subpopulations. Data represents mean ± SD from three experiments in duplicate, *p≤0.05.