Met signature segmentation of cell line model and human breast cancer patients’ data sets.

<p>(A) Cells from six human breast cancer cell lines (MDA231, Hs578T, BT549, MCF10, MCF7 and T47D) were incubated with purified HGF/SF labeled with biotin by a protein biotinylation kit and allowed to bind for 30 min. Cells were then fixed with 4% Paraformaldehyde, permeablized, and stained with Streptavidin-coupled QDot585. Fluorescence levels calculated by image analysis using MICA image analysis software, p<0.0001. (B) Met canonical pathway score calculated by measuring the average mRNA levels of all Met canonical pathway genes (after normalization to average = 0, stdev = 1 per-gene) in high-Met (MDA231, Hs578T and BT549) as compared to the low-Met (MCF10, MCF7 and T47D) samples, p<0.0001. A gray box denotes high Met cell line samples and a black box denotes low Met cell line samples. (C) Hierarchical clustering division of breast cancer cell lines samples using Met kinetic signature genes.