MHC-II-, CD19- and CD68-positive cells in PC-3 tumors and lymph node metastases.

<p>(a) Staining of 100 µm PC-3-RFP tumor, LN and RN sections with anti-MHC-II antibody 47 days post tumor cell implantation. (b, c, d) Amount of MHC-II-, CD19- and CD68-positive cells in PC-3-RFP tumors, LNs and RNs. 10 µm cryosections of tumors, LNs and RNs of 8 mice 50 days post implantation were stained with anti-MHC-II, anti-CD19 and anti-CD68 antibody, respectively. Images of whole sections were taken and the area of a section positive for MHC-II, CD19 or CD68 cells was measured using ImageJ. Two sections were analyzed per sample. Below the diagrams are representative images of cryosections of a renal lymph node metastasis stained for MHC-II, CD19 and CD68. Scale bars represent 500 µm (a) and 1 mm (b, c, d).