PfASP shows a dual localisation to both the tight junction and merozoite apex during erythrocyte invasion.

<p>Widefield 3D imaging of ASP-HA merozoites labeled with anti-HA, anti-PfRON4 and DAPI showing early invasion events (<b>A</b>) and two classes of mid/late invasion (<b>B, C</b>) distributions seen during invasion. Scale bar = 5 µm. 3D reconstruction with 0.2 µm grid intervals.