Amino acids 171–240 of recombinant PorA exhibit LamR-binding activity.

<p>(A) Schematic showing the recombinant derivatives of <i>N. meningitidis</i> MC58 PorA utilized in this study and a summary of their respective LamR-binding activities. L1–L8 denotes the eight extra-cellular loops based on the model of van der Ley <i>et al. </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046233#pone.0046233-vanderLey1" target="_blank">[23]</a>. (B) Binding of LamR to the solid phase antigens PorA^20–392, PorA^20–170, PorA^20–258 and PorA^20–332. (C) Binding of LamR to PorA^20–392, PorA^95–170 and PorA^150–258. (D) Binding of LamR to PorA^20–392, PorA^Δ58–94, PorA^Δ125–149 and PorA^Δ169–240. Data shown in each panel are means from three independent experiments; in each experiment each sample was tested in triplicate. ** <i>p</i><0.01 compared to binding with matching PorA^20–392 samples. Error bars indicate SEM.