PAH activity in lysates of <i>L. pneumophila</i> 130b.

<p>PAH activity measured without (A) and with (B) the addition of 100 µM Fe(II) in the assay in lysates from wild-type (WT) cultures grown in BYE medium with (BYÊhigh Fe; white bars) and without (BYÊlow Fe; black bars) standard (1.3 mM) FeCl₃ supplement (see text for details). The data presented are the means and SD from triplicate measurements of duplicate cultures for each condition. The activity of a lysate of a culture of <i>phhA</i> mutant grown in BYÊhigh Fe is also shown in (B). Differences between conditions were found to be significant (P<0.01) with respect to WT in BYÊhigh Fe, in both (A) and (B).