Characterization of MEF^PTEN−/−.

<p>(<b>A</b>) <i>Western blot analyses of PTEN, Akt, p-Ser⁴⁷³Akt and p-Thr³⁰⁸Akt</i> Immunoblotting of PTEN, Akt, p-Ser⁴⁷³Akt and p-Thr³⁰⁸Akt was carried out on whole cell lysates of MEF^WT and MEF^PTEN−/−. The loss of PTEN in MEF^PTEN−/− resulted in hyperactivation of Akt on both phosphorylation sites without affecting the expression of total Akt. The respective loading controls of α-tubulin were included. (<b>B</b>) <i>Rate of cell proliferation</i> This was measured by the trypan-blue exclusion assay. The MEF^PTEN−/− has a significantly higher rate of proliferation compared to MEF^WT from days 1–4 of culture. # <i>p</i><0.005 for n = 3.