Characterization of high dose zymosan peritonitis. A)

<p>Representative flow-cytometric density plots showing the recruitment of inflammatory cells and the clearance of fluorescent (FITC)-labelled zymosan particles. The examples shown are taken from 18 hours after the administration of zymosan. Cells were identified as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045781#s2" target="_blank">methods</a>. The panel on the left shows the gating of Ly-6B⁺ cells, which includes (right) Ly-6G^high neutrophils and Ly-6G⁻ monocytes and MØ. Both neutrophils and monocytes/MØ exhibit association with FITC-labeled zymosan at this time point (right panel). <b>B)</b> Graphical representation of the number of neutrophils (left) and all types of monocyte (Mo) and MØ combined (right) in the peritoneal cavity before and after acute zymosan peritonitis. n = 3 129S6/SvEv mice per group and is representative of 2 independent experiments. Data is shown as mean±SEM and cells were isolated from naïve animals (0 hours), and challenged mice 4, 18, 72 and 168 hours after induction of peritonitis. <b>C)</b> Graphical representation of the percentage of neutrophils and Mo/MØ present that are associated with zymosan, scored by flow-cytometry as indicated in (A) above. <b>D)</b> Photomicrograph, which is representative of neutrophils associated with zymosan in a 4 hour inflammatory infiltrate. Those neutrophils that are associated tend to have multiple particles, but only represent a minority of the total neutrophils present at this time (see (C) above).