Pump-probe absorption spectroscopy of enzyme-denatured POR-Pchlide-NADPH samples.

<p>Spectra are shown after photoexcitation with a laser pulse centred at ∼475 nm. The main panels show transient absorption difference spectra at delay times of 2, 16, 516 and 2980 ps after excitation for POR-Pchlide-NADPH samples that were kept in the dark (A), illuminated for 2 mins (B) and illuminated for 4 mins (C) prior to enzyme denaturation. The insets show the respective kinetic transients at 675 nm (black circles) with a fit of the data to 3 exponentials (solid line).