Pump-probe absorption spectroscopy of samples containing a mixture of 50% Pchlide and 50% Chlide in methanol after photoexcitation with a laser pulse centred at ∼460 nm.

<p>The main panel show transient absorption difference spectra at delay times of 2, 16, 516 and 2980 ps after excitation. The inset shows the residuals at 675 nm for a mixture of 50% Pchlide and 50% Chlide in methanol and H₂O after subtraction of the sum of the ‘Pchlide only’ and ‘Chlide only’ data from the actual time-resolved difference spectra.