Co-expression of hypoxia, EMT and CSC markers in pancreatic cancer tissues.

<p>(<b>A</b>) Double immunofluorescence stainings of tumor samples from patients with pancreatic cancer. Nuclear expression of HIF-1α (green, arrow) and membrane expression of E-cadherin (red, arrow) or Slug (red, arrow) is shown. Yellow color on merged images indicates co-expression of E-cadherin or Slug in HIF-1α-positive cells. (<b>B</b>) Membrane expression of E-cadherin (red), cytoplasmic expression of Vimentin (green) along with Dapi-staining of nuclei (blue). White squares indicate Vimentin-positive cells within the tumor mass that are negative for E-cadherin or <i>vice versa</i>. Bar: 100 μm. Twofold magnifications of the areas surrounded by white squares are shown on the left. (<b>C</b>) Nuclear expression of HIF-2α and membrane expression of CD133 is shown as single staining and as merged staining in which the yellow color indicates double-positivity.