Knockdown of TCF7L2 promotes elevations in blood glucose levels in C57BL/6 mice.

<p>A) Western blot analysis showing protein expression levels of TCF7L2 M, TCF7L2 S, and TCF7L2 E in livers of high-fat diet-fed or normal chow diet-fed (top), and <i>db/db</i> or C57BL/6 mice (bottom). B) 16 h fasting glucose levels (top, left), 6 h fasting glucose levels (top, right), or feeding glucose levels (bottom) from 8-week-old C57BL/6 male mice that were infected with Ad-US (<i>n</i> = 7) or Ad-shTCF7L2 (<i>n</i> = 6). C) Q-PCR analysis showing effects of Ad-US (<i>n</i> = 3) or Ad-shTCF7L2 (<i>n</i> = 4) on hepatic expression of PEPCK, G6Pase, and PGC1α in C57BL/6 mice fasted for 6 h. D) Q-PCR analysis showing effects of Ad-US (<i>n</i> = 4) or Ad-shTCF7L2 (<i>n</i> = 4) on hepatic expression of G6Pase, PEPCK, and Lipin1 in C57BL/6 mice under feeding conditions. E) Western blot analysis showing effects of Ad-shTCF7L2 on insulin signaling pathway in mice. C57BL/6 mice infected with either Ad-US or Ad-shTCF7L2 for 5 days were fasted for 6 h, and then were given a bolus of insulin or saline for 10 min before being sacrificed. Data in B) represent mean ± SEM, and data in C) and D) represent mean ± SD (*;<i>P</i><0.05, **;<i>P</i><0.005, ***;<i>P</i><0.0005, t-test).