<p>(A) Transformation efficiency of the <i>B. amyloliquefaciens</i> TA208 strain with various shuttle plasmids prepared from the <i>E. coli</i> TOP10, EC135 and the EC135 harboring pM.Bam. (B) Transformation efficiency of the <i>B. cereus</i> ATCC 10987 strain with various shuttle plasmids prepared from the <i>E. coli</i> TOP10, EC135 and the EC135 harboring pM.Bce. Transformation efficiencies shown are averages of at least three replicates ± SD. * Not Detected.</p
Most plasmids replicate only within a particular genus or family.Here we describe an engineered high...
(1) The low residual transforming activity in preparations of monomeric, supercoiled, circular (CCC)...
395-400The efficiencies of different transformation methods of E. coli DH5α strain, induced by sever...
<p>Chemically competent <i>E</i>. <i>coli</i> DH5α and JM109 cells were transformed with 100pg μL<su...
<p>Transformation with pDM1 plasmid DNA isolated from CNCM I-2494, <i>E. coli</i> pWSK29, <i>E. coli...
<p>(A) <i>E. coli</i> was transformed with plasmids pBAV1K-T5-<i>gfp</i>, pLZ12-T5-<i>gfp</i>, pGK12...
Factors that affect he probability of genetic transformation f Escherichia coli by plasmids have bee...
Additional file 2: Figure S2. Improvements in transformation efficiency are linked to the type of pl...
<p>Plasmid pBSV2G DNA is used to transform <i>B</i>. <i>burgdorferi</i> strains by electroporation. ...
<p>Transformation efficiencies upon selection with the indicated antibiotics on 7H11-OADC agar are e...
<p>Graphs A-C are for pSK236 efficiency, D-F are for pEPSA5 efficiency. A and D pertain to MW2 (CC1)...
Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et a...
DNA manipulation routinely requires competent bacteria that can be made using one of numerous method...
Four shuttle vectors (pMIG 1, 2, 2H and 3) have been constructed based on the broad host‐range plasm...
An efficient protoplast transformation system was established for Bacillus stearothermophilus NUB362...
Most plasmids replicate only within a particular genus or family.Here we describe an engineered high...
(1) The low residual transforming activity in preparations of monomeric, supercoiled, circular (CCC)...
395-400The efficiencies of different transformation methods of E. coli DH5α strain, induced by sever...
<p>Chemically competent <i>E</i>. <i>coli</i> DH5α and JM109 cells were transformed with 100pg μL<su...
<p>Transformation with pDM1 plasmid DNA isolated from CNCM I-2494, <i>E. coli</i> pWSK29, <i>E. coli...
<p>(A) <i>E. coli</i> was transformed with plasmids pBAV1K-T5-<i>gfp</i>, pLZ12-T5-<i>gfp</i>, pGK12...
Factors that affect he probability of genetic transformation f Escherichia coli by plasmids have bee...
Additional file 2: Figure S2. Improvements in transformation efficiency are linked to the type of pl...
<p>Plasmid pBSV2G DNA is used to transform <i>B</i>. <i>burgdorferi</i> strains by electroporation. ...
<p>Transformation efficiencies upon selection with the indicated antibiotics on 7H11-OADC agar are e...
<p>Graphs A-C are for pSK236 efficiency, D-F are for pEPSA5 efficiency. A and D pertain to MW2 (CC1)...
Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et a...
DNA manipulation routinely requires competent bacteria that can be made using one of numerous method...
Four shuttle vectors (pMIG 1, 2, 2H and 3) have been constructed based on the broad host‐range plasm...
An efficient protoplast transformation system was established for Bacillus stearothermophilus NUB362...
Most plasmids replicate only within a particular genus or family.Here we describe an engineered high...
(1) The low residual transforming activity in preparations of monomeric, supercoiled, circular (CCC)...
395-400The efficiencies of different transformation methods of E. coli DH5α strain, induced by sever...