Construct and hAR protein expression in MHC-hAR mice.

<p>(<b>A</b>) hAR construct design employed in this study is presented. The α-MHC promoter was used to drive hAR cDNA expression. The construct scheme indicate exons that are numbered (black boxes) along with poly(A) site (PA). (<b>B</b>) Cardiac-specific hAR expression are presented for. Western blotting was performed in 3-month-old MHC-hAR male mice using polyclonal human AR antibody. Total protein (30 µg) from adipose, heart, muscle and liver tissues of MHC-hAR and their littermate controls was analyzed. GAPDH antibody is shown as a control.