Characterization of the LasR-regulated small RNAs Lrs1 and Lrs2.

<p>(A) Growth (lines) and expression of the <i>lrs1</i> or <i>lrs2</i> reporter constructs (bars) in wild-type or Δ<i>lasR P. aeruginosa</i> PA14 are shown. (B) Role of Hfq in controlling the levels of Lrs1 and Lrs2 sRNAs. Gel shift analyses of sRNAs interaction with increasing amounts of purified Hfq are shown. Lane 5 in both panels contains, in addition to labeled probe, a 100-fold excess of unlabeled Lrs1 or Prrf1 RNA. (C) Lower panels show RT-PCR analyses of Lrs1 and Lrs2 expression in <i>P. aeruginosa</i> wild-type and Δ<i>hfq</i>. Prrf1 and RsmZ were used as positive and negative controls, respectively, for sRNAs interacting and not interacting with Hfq.