Zyxin partially co-distributes with dSH2 in focal adhesions and is recruited from zyxin-enriched fibrillar adhesion upon EGF treatment.

<p>MKN1 cells were co-transfected with constructs encoding RFP-zyxin and CFP-dSH2 (depicted in false green color). Note that both co-localize only partially in the more central parts of focal adhesions in the cell periphery (A, B). This is further highlighted by the line diagrams in A’ that were recorded along the arrow in A. In contrast, more centrally located focal adhesions and large fibrillar adhesions are predominantly positive for zyxin and contain very little dSH2 label (C, D). (D, D’) is taken from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045280#pone.0045280.s010" target="_blank">Video S10</a> and depicts a cell immediately before and 59 min after addition of 5 ng/ml EGF. Note the reduction of the very large zyxin-positive plaque-like areas (arrowheads in D) and the appearance of novel contacts in newly-formed lamella (arrowhead in D’). Bars, 2 µm in A, B; 5 µm in C, D.