TLR7 ligand treatment selectively impaired dendritic cell and not T cell function.

<p>(A) CFSE-labeled CD4⁺ T cells were prepared from WT or <i>Tlr7^−/−</i> mice immunized with OVA/CFA. WT or <i>Tlr7^−/−</i> BMDCs were incubated with these primed CD4⁺ T cells with OVA (100 µg/mL) in the presence or the absence of 1V136 (1 µM) for five days. T cell proliferation was monitored by flow cytometry. Representative plots are shown from three experiments. (B) The culture supernatants were assessed for IFN-γ levels by ELISA. The data shown are representative of two independent experiments. p<0.05 by Student <i>t</i> test compared to vehicle control.