Repression of somatic gene expression does not depend on DNA methylation in PGCs.

<p>(A) Bisulfite sequencing analysis of the flanking regions of <i>Hoxa1</i>, <i>Hoxb1</i>, and <i>Gfap</i> was performed using epiblasts (E6.0), epiblast stem cells (EpiSCs), and PGCs (E13.5), showing hypomethylation in PGCs. (B) Bisulfite sequencing analysis of the regulatory region of <i>Gfap</i> and (C) quantitative RT-PCR analysis of the <i>Gfap</i> expression were performed using ES cells with or without <i>Dnmt1</i> knockdown treatment (<i>Dnmt1</i> KD/Con KD).