Enrichment of DNA sequences that bind to AGR2-GST-beads.

<p>a) Flow cytometry assay to monitor the binding of a selected pool with AGR2 (target protein) and GST (control protein). The red curve represents the background binding of unselected library. For the target protein AGR2, there was an increase in binding capacity of the pool as the selection progressed, while there was no observable change for the control protein GST. The final concentration of the selected pool in binding buffer was 100 nM. b) Determination of the dissociation constant of the enriched library and unselected library to AGR2. c) Fluorescence measurements to determine the dissociation constant of selected aptamer C14B. Two negative controls, GST-beads and GSH-beads, were carried out. The data were the average of triplicate experiment results.