Gap gene expression in pair-rule gene knockdown embryos at stage 4.

<p>All embryos are oriented with anterior to the left and dorsal side up. Scale bars are 200 µm. (A) <i>Am-gt</i> is expressed in an anterior domain (arrowheads) and a posterior stripe (line). (B) is reduced in <i>Am-eve</i>^RNAi embryo showing reduction in <i>Am-gt</i> expression into a broad abdominal domain (line). (C) <i>Am-run</i>^RNAi embryos stained for <i>Am-gt</i> RNA displaying loss of the posterior stripe of <i>Am-gt</i> RNA(asterisk), and expansion of the anterior domain towards the posterior, as well as some retraction of staining from anterior and dorsal regions of the embryo (arrowheads). (D) <i>Am-h</i>^RNAi embryo stained for <i>Am-gt</i> RNA displaying reduction of the anterior domain (arrowheads). (E) <i>Am-ftz</i>^RNAi embryo stained for <i>Am-gt</i> RNA indicating loss of the anterior domain, and disruption of the posterior stripe. (F) <i>Am-kr</i> is expressed in a broad domain in central regions of the embryo. (G) <i>Am-kr</i> expression in an <i>Am-eve</i>^RNAi embryo showing reduction in intensity of staining but expansion of the Am-kr domain to the entire embryo excepting the posterior pole. (H) The <i>Am-kr</i> expression domain in an <i>Am-run</i>^RNAi embryo is shifted slightly towards the posterior (arrowheads). (I) <i>Am-kr</i> expression in an <i>Am-h</i>^RNAi embryo is slightly shifted to the anterior of the normal expression domain. (J) <i>Am-kr</i> RNA stained <i>Am-ftz</i>^RNAi embryo showing the Am-kr expression domain (arrowheads) shifted toward the posterior. (K) <i>Am-tll</i> RNA is expressed in an anterior triangular domain (arrow) and a posterior cap (asterisk). (L). <i>Am-eve</i>^RNAi embryo stained for <i>Am-tll</i> RNA showing loss of the anterior domain of <i>Am-tll</i>. (M). <i>Am-tll</i> RNA expression in an <i>Am-run</i>^RNAi embryos showing loss of the anterior domain of expression. (N) <i>Am-h</i>^RNAi embryostained for <i>Am-tll</i> RNA showing no difference from control embryos. (O) <i>Am-ftz </i>^RNAi embryo stained for <i>Am-tll</i> RNA showing no anterior expression domain and weak staining in the posterior cap.