Ad-A20 administration mediated protection against CVB3-induced myocarditis.

<p>Mice were intravenously injected with saline or 3×10⁹ pfu of either Ad-A20 or Ad-LacZ 2 days before 10³ TCID₅₀ dose of CVB3 infection. Mice without infection were as control group. (A and B) The body weight change (A) and survival rate (B) were respectively monitored daily until day 7 and day 10 post-infection. (C) Serological indices of myocarditis, the activity of CK, CK-MB and cTnI in mouse serum were detected on day 7 post-infection. (D<b>)</b> Paraffin sections of heart tissues were prepared on day 7 and cardiac inflammation was revealed by H&E staining (magnification:×200). The severity of myocarditis was scored by a standard 0–4 scale according to the foci of mononuclear infiltration and myocardial necrosis. Individual experiments were conducted 3 times with similar results, with 1 representative shown for each group. Data show the means±SEM of 6 mice per group. *, <i>P</i><0.05; **, <i>P</i><0.01; N.D., not detected.