Effect of Anr in regulation of <i>fabAB</i> expression.

<p><b>A</b>) <i>anr</i> is responsible for activation of <i>fabA</i>′-′<i>lacZY</i> transcription in <i>E. coli</i>. The restriction maps shown here are from cloned chromosomal DNA fragments isolated from blue, putative activator-expressing colonies. Plasmids isolated from ten blue colonies were sequenced by using M13 forward and reverse primers to verify the presence of the indicated genes. <b>B</b>) Effects of <i>anr</i> on <i>fabA</i> expression in <i>P. aeruginosa</i>. β-galactosidase activities were measured in wild-type and Δ<i>anr</i> PAO mutants containing a chromosomally integrated p<i>fabA</i>′-<i>lacZ</i> fusion. Columns: 1, PAO1 (wild-type); 2, PAO1010 (PAO1 Δ<i>anr</i>); 3, PAO1010 with pPS1684 (pUCP20 with <i>anr</i>⁺); 4, PAO1010 with pPS1682 (pVLT35 with <i>anr</i>+) in the absence of IPTG; 5, PAO1010 with pPS1682 in the presence of IPTG.