Separation and comparison of saliva protein fractions from <i>M. viciae</i> collected in SE – and CW-diet.

<p>Soluble and non-soluble protein fraction of saliva concentrate collected from SE-diet and CW-diet were separated in a 10% separation gel. Gels were silver stained. Lane 1 of each gel contains marker proteins Molecular weights (kDa) are given on the left. <b>A</b>) The soluble fraction from SE-diet shows 45 protein bands while in samples from CW-diet only 6 protein bands are detected. All latter protein bands match bands from the SE-diet but have a much lower abundance. <b>B</b>) Non-soluble fraction samples have fewer protein bands in CW-diet (lane 3, n = 66) than in SE-diet (lane 2, n = 87). Matching bands of lanes 2 and 3 show comparable intensities. The most intense protein bands (MWs are given in <b>B</b>) of the non-soluble fraction from CW-diet are used as reference proteins for this saliva fraction and are labeled with their MWs. Protein bands with corresponding MW in other lanes are marked with red asterisks. Protein bands of insoluble fraction that correspond to soluble fraction from SE-diet (<b>A</b>) are labeled with green asterisks and MWs are given in <b>A</b>. <b>C</b>) To discriminate protein bands of non-soluble fraction (red graph) from those of soluble fraction (green graph), soluble fraction from SE-diet (<b>A</b>, lane 2) was compared with non-soluble fraction from CW-diet (<b>B</b>, lane 3). The relative pixel intensity value (arbitrary units) is plotted against the retardation factor (Rf-value). Most intense proteins from non-soluble fraction (MWs are given), all of which have a relative pixel intensity >50, show a coincidence with 6 protein bands in the soluble fraction collected from SE-diet (red asterisks). Specific proteins from soluble fraction with a relative intensity of more than 50 (RF-value of approx. 0.2 (MWs in <b>A</b> and green asterisks in <b>B</b>) and higher 0.57), are only slightly visible in non-soluble fraction from CW-diet (<b>C</b>).