Representative EMSA and corresponding densitometric analysis show the effects of dec-ODN released from LPP_PEI or naked dec-ODN decoy on NF-κB/DNA binding activity in LPS-stimulated NCI-H292 cells at 24 and 72 h.

<p>Densitometric data are expressed as mean ± S.E.M. of three separate experiments. °°°p<0.001 vs. unstimulated cells; ***p<0.001 vs. LPS alone. (B-C) Characterization of NF-κB/DNA complex was performed on nuclear extracts from LPS-stimulated NCI-H292 cells at 24 h (W.T. = <i>wild tipe</i> dec-ODN; Mut. = <i>scramble</i> dec-ODN). Plots in (A,B,C) are from a single experiment and are representative of three separate experiments.