Loss of the Sas3 and Gcn5 H3 acetyltransferases modestly alters Isw1a recruitment to chromatin.

<p>(A) Schematic representation of the <i>PYK1</i> locus and positions of primer pairs used for ChIP analysis in (B), (E) and (F). Ioc3-Myc occupancy was assayed by ChIP in wild-type and <i>gcn5</i>Δ <i>sas3</i> cells grown in SC medium at 34°C over the <i>PYK1</i> gene (B), at promoter (C) and 5′ coding regions (D) of <i>RPL10</i>, <i>CDC25</i> and <i>UBP7</i> genes. (E) Histone H3K14 acetylation occupancy was analyzed by ChIP over the <i>PYK1</i> gene in wild-type and <i>gcn5</i>Δ <i>sas3</i> cells grown in SC medium at 34°C. (F) Histone H3 occupancy over the <i>PYK1</i> gene was assayed by ChIP in wild-type, <i>gcn5</i>Δ <i>sas3</i>, and <i>gcn5</i>Δ <i>sas3 ioc3Δ</i> cells grown in SC medium at 34°C. Ioc3-Myc occupancy was normalized to the telVI control region. H3K14Ac and H3 levels were normalized to the promoter region of <i>PYK1</i>. The values represent the means from two or more independent experiments, with error bars reflecting standard deviations.