Quantitative analysis of <i>in vivo</i> CAT expression from co-transfection of transcription factor siRNA vector and AeSCP-2 promoter/CAT constructs (1∶1 ratio).

<p>In the promoter/CAT group, <i>hsp70</i> short promoter vector plasmid was added in at 1∶1 ratio to normalize the total amount of promoter/CAT each group received. Larvae were synchronized on Day 1 2^nd instar, heat shocked at 37°C for 24 hours on Day 1 of 2^nd and 4^th instar, respectively. Day 1 4^th instar larval samples were taken after the 2^nd heat shock-treatment. (<b>A</b>) The midgut samples from 4^th instar larvae (30 larvae/sample) were taken at 24 hour post 3^rd molt. (<b>B</b>) The carcass samples were from the same larvae as the midgut sampling. (<b>C</b>) Larval midgut endogenous transcription of <i>AeSCP-2</i> in −1.3 kb/CAT and siRNA co-transfected larvae. Ten synchronized 24 h 4^th instar larvae were pooled from each batch of the co-transfection experiment (in panel A, −1.3 kb). Relative <i>AeSCP-2</i> mRNA levels (vs. <i>Actin-1</i>) from each sample were determined via RT-qPCR. Mean and standard deviation are shown (N = 3). * Indicating significantly different (<i>p</i><0.05) from the promoter/CAT control.