Inactivation of the Isw1a complex in the <i>gcn5</i>Δ <i>sas3</i> mutant does not significantly alter nucleosome positioning at the <i>PYK1</i> gene.

<p>(A) A nucleosome scanning assay was performed on chromatin from WT, <i>gcn5</i>Δ <i>sas3</i>, and <i>gcn5</i>Δ <i>sas3 ioc3Δ</i> cells grown in SC medium at 34°C. Mononucleosomal DNA was purified and analyzed by real-time qPCR using seventeen overlapping primer pairs spanning the promoter and 5′ coding region of <i>PYK1</i>. Values are expressed as percentage of input, and normalized to the <i>PHO5</i> TATA region <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002994#pgen.1002994-Wu1" target="_blank">[77]</a>. The values represent the means from three independent experiments, with error bars reflecting standard deviations. (B) Diagram of the <i>PYK1</i> locus indicates the positions of nucleosomes (gray ovals) extrapolated from the MNase protection assay.